Real-Time PCR Measurement by Fluorescence Anisotropy
نویسندگان
چکیده
منابع مشابه
Real-time PCR measurement by fluorescence anisotropy.
We have developed an instrument for monitoring real-time PCR using fluorescence anisotropy, enabling an assay chemistry in which the fluorescence from a labeled primer elucidates amplification. The instrument holds the sample temperature constant to within +/-0.03 degrees C during measurement in the extension phase of each PCR cycle and achieves 0.116 mP FA resolution. Primer conjugation with A...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2005
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac050323j